Publications

2016

  • R. Toy and K. Roy. Engineering Nanoparticles to Overcome Barriers to Immunotherapy. Bioengineering & Translational Medicine, 2016. DOI: 10.1002/btm2.10005
  • K.H. Roh and K. Roy. Engineering approaches for regeneration of T lymphopoiesis. Biomater Res. 2016 Jun 29;20:20. doi: 10.1186/s40824-016-0067-1. eCollection 2016. PubMed
  • K.H. Roh, R.M. Nerem, and K. Roy. Biomanufacturing of Therapeutic Cells: State of the Art, Current Challenges, and Future Perspectives. Annu Rev Chem Biomol Eng. 2016 Jun 7;7:455-78. doi: 10.1146/annurev-chembioeng-080615-033559. PubMed
  • M.R. Nelson and K. Roy. Bone-marrow mimicking biomaterial niches for studying hematopoietic stem and progenitor cells. J. Mater. Chem. B, 2016,4, 3490-3503. DOI: 10.1039/C5TB02644J

2015

  • J. Leleux, A. Atalis, and K. Roy. Engineering immunity: Modulating dendritic cell subsets and lymph node response to direct immune-polarization and vaccine efficacy.J Control Release. 2015 Dec 10;219:610-21. doi: 10.1016/j.jconrel.2015.09.063. Epub 2015 Oct 20.
  • R. Agarwal, P. Jurney, M. Raythatha, V. Singh, S.V. Sreenivasan, L. Shi, K. Roy. Effect of shape, size, and aspect ratio on nanoparticle penetration and distribution inside solid tissues using 3D spheroid models.Adv Healthc Mater. 2015 Oct 28;4(15):2269-80. doi: 10.1002/adhm.201500441. Epub 2015 Sep 16.

2014

  • V. Singh, R. Agarwal, K. Roy., L. Shi, S.V. Sreenivasan. Scalable Fabrication of Ultra-Soft Shape Specific Nanocarriers for Diagnostics and Drug Delivery. Journal of Micro and Nano-Manufacturing. Epub 2014 Oct; doi:10.1115/1.4028896. ASME
  • S. Li, K.K. Hirschi, K. Roy. Induced Pluripotent Stem Cells for Regenerative Medicine, Annu Rev Biomed Eng. 2014 Jul;16:277-94. doi: 10.1146/annurev-bioeng-071813-105108. ASME
  • P. Pradhan, H. Qin, J.A. Leleux, D. Gwak, I. Sakamaki, L.W. Kwak, K. Roy. The effect of combined IL10 siRNA and CpG ODN as pathogen-mimicking microparticles on Th1/Th2 cytokine balance in dendritic cells and protective immunity against B cell lymphoma. Biomaterials. 2014 Jul; 35(21):5491-504. doi: 10.1016/j.biomaterials.2014.03.039. PubMed
  • A. Purwada, K. Roy, A. Singh. Engineering vaccines and niches for immune modulation, Acta Biomaterialia. 2014, Apr:10(4):1728-1740.doi:10.1016/j.actbio.2013.12.036. PubMed
  • J.S. Lewis, K. Roy, B.G. Keselowsky. Materials that harness and modulate the immune system, MRS Bulletin 39 (01), 25-34. CJO
  • I. Fernandez, T.P. Ooi, K. Roy. Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous notch and tetramer-TCR signaling. Stem Cells. 2014 Jan; 32(1). doi: 10.1002/stem.1512. PubMed

2013

  • P. Jurney, R. Agarwal, V. Singh, K. Roy., S.V. Sreenivasan, L. Shi. Size-Dependent Nanoparticle Margination and Adhesion Propensity in a Microchannel. Journal of Nanotechnology in Engineering and Medicine. 2013 Nov;4(3). doi: 10.1115/1.4025609. ASME
  • R. Agarwal, V. Singh, P. Jurney, S.V. Sreenivasan, L. Shi, K. Roy. Cells Prefer Hydrogel Nanodiscs over Nanorods and Use Shape-specific Uptake Mechanisms. Proc Natl Acad Sci USA. 2013 Oct;110(43):17247-52. doi: 10.1073/pnas.1305000110. PNAS
  • R. Agarwal, K. Roy. Intracellular Delivery of Polymeric Nanocarriers: A Matter of Size, Shape, Charge, Elasticity and Surface Composition. , Therapeutics Delivery. 2013 Jun;4(6):705-23. doi: 10.4155/tde.13.37. Pubmed
  • J. Leleux, K. Roy. Micro and nanoparticle-based delivery systems for vaccine immunotherapy: an immunological and materials perspective. Adv Healthc Mater. 2013 Jan;2(1):72-94. doi: 10.1002/adhm.201200268. Pubmed

2012

  • I. Fernandez, K. Fridley, D. Arasappan, R. Ambler, P.W. Tucker, K. Roy. Gene expression profile and functionality of ESC-derived Lin-ckit+Sca-1+ cells are distinct from Lin-ckit+Sca-1+ cells isolated from fetal liver or bone marrow. PLoS One. 2012;7(12) doi: 10.1371/journal.pone.0051944. PubMed
  • P. Wanakule, G.W. Liu, A.T. Fleury, K. Roy. Nano-inside-micro: Disease-responsive microgels with encapsulated nanoparticles for intracellular drug delivery to the deep lung. Journal of Controlled Release. 2012 Jul;162(2):429-437. doi: 10.1016/j.jconrel.2012.07.026. PubMed
  • R. Agarwal, V. Singh, P. Jurney, L. Shi, S.V. Sreenivasan, K. Roy. Scalable imprinting of shape- specific polymeric nanocarriers using a release layer of switchable water solubility. ACS Nano. 2012 Mar; 6(3):2524-2531. doi: 10.1021/nn2049152. PubMed
  • P. Wanakule, K. Roy. Disease-Responsive Drug Delivery: The Next Generation of Smart Delivery Devices. Current Drug Metabolism, Volume 13, Number 1, January 2012, pp. 42-49(8). PubMed

2011

  • L. Nguyen, A. Kudva, N. Saxena and K. Roy. Engineering articular cartilage with spatially varying matrix composition and mechanical properties from a single stem cell population using a multi- layered hydrogel. Biomaterials. 2011 Oct; 32(29):6946-52. doi: 10.1016/j.biomaterials.2011.06.014. PubMed
  • A. Singh, H. Qin, I. Fernandez, J. Wei, J. Lin, L. W. Kwak and K. Roy. An injectable synthetic immune-priming center mediates efficient T-cell class switching and T-helper 1 response against B cell lymphoma. Journal of Controlled Release. 2011 Oct;155(2):184-92 doi: 10.1016/j.jconrel.2011.06.008. PubMed
  • M Caldorera-Moore, MK Kang, Z Moore, V Singh, SV Sreenivasan, L Shi, R Huang and K Roy. Swelling behavior of nanoscale, shape- and size-specific, hydrogel particles fabricated using imprint lithography. Soft Matter. 2011; 7:2879-2887. RSC
  • J Lin, I Fernandez, K Roy. Development of Feeder-Free Culture Systems for Generation of ckit+sca1+ Progenitors from Mouse iPS Cells. Stem Cell Rev. 2011 Sep;7(3):736-47. doi: 10.1007/s12015-010-9215-5. (J Lin and I Fernandez contributed equally to this work). PubMed

2010

  • K Fridley, I Fernandez, M-T A Li, RB Kettlewell, K Roy. Unique Differentiation Profile of Mouse Embryonic Stem Cells in Rotary and Stirred Tank Bioreactors. Tissue Engineering Part A. 2010 Nov;16(11):3285-98. doi: 10.1089/ten.TEA.2010.0166. PubMed
  • B Ghosn, A Singh, M Li, AV Vlassov, C Burnett, N Puri, K Roy. Efficient gene silencing in lungs and liver using imidazole-modified chitosan as a nanocarrier for small interfering RNA. Oligonucleotides.2010 Jun;20(3):163-72. doi: 10.1089/oli.2010.0235. PubMed
  • J Lin, H Nie, PW Tucker, K Roy. Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8(+) T Cells from Embryonic Stem Cells and Thymic Progenitors. Tissue engineering Part A. 2010 Sep;16(9):2709-20. doi: 10.1089/ten.TEA.2009.0707. PubMed
  • M Caldorera-Moore, N Guimard, L Shi, K Roy. Designer nanoparticles: incorporating size, shape and triggered release into nanoscale drug carriers. Expert Opin Drug Deliv. 2010 Apr;7(4):479-95. doi: 10.1517/17425240903579971. PubMed
  • I Kadiyala, Y Loo, K Roy. J Rice, KW Leong. Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes. Eur J Pharm Sci. 2010 Jan 31;39(1-3):103-9. doi: 10.1016/j.ejps.2009.11.002. PubMed
  • B Ghosn, AL van de Ven, J Tam, A Gillenwater, KV Sokolov, R Richards-Kortum, K Roy. Efficient mucosal delivery of optical contrast agents using imidazole-modified chitosan. J Biomed Opt. 2010 Jan-Feb;15(1):015003. doi: 10.1117/1.3309739. PubMed

2009

  • A Singh, S Suri, K Roy. In-situ crosslinking hydrogels for combinatorial delivery of chemokines and siRNA-DNA carrying microparticles to dendritic cells. Biomaterials. 2009 Oct;30(28):5187-200. doi: 10.1016/j.biomaterials.2009.06.001. PubMed

2008

  • A. Singh, H. Nie, B. Ghosn, H. Qin, L. W. Kwak, K. Roy. Efficient modulation of T cell response by dual-mode, single carrier delivery of cytokine-targeted siRNA and DNA vaccine to antigen presenting cells. Molecular Therapy. 2008 Dec;16(12):2011-21. doi: 10.1038/mt.2008.206. PubMed
  • E. Dawson, G. Mapili, K. Erickson, S. Taqvi and K. Roy. Biomaterials for Stem Cell Differentiation. Adv Drug Delivery Review. 2008 Jan 14;60(2):215-28. PubMed
  • B. Ghosn, S. Kasturi and K. Roy. Enhancing Polysaccharide-mediated Delivery of Nucleic Acids Through Functionalization with Secondary and Tertiary Amines. Current Topics in Medicinal Chemistry. 2008;8(4):331-40. PubMed
  • L.C. Glangchai, M.C. Moore, L. Shi and K. Roy. Nanoimprint Lithography Based Fabrication of Shape-specific, Enzymatically-Triggered Smart Nanoparticles. Journal of Controlled Release. 2008 Feb 11;125(3):263-72. PubMed

2007

  • L.H. Han, G. Mapili, S. Chen, and K. Roy. Projection micro-printing of 3-dimensional scaffolds for tissue engineering. Journal of Manufacturing Science and Engineering, 2007
  • L. Brannon-Peppas, B. Ghosn, K. Roy, K. Cornetta. Encapsulation of nucleic acids and opportunities for cancer treatment. Pharm Res. 2007 Apr;24(4):618-27. PubMed

2006

  • H. Liu, J. Lin and K. Roy. Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells. Biomaterials. 2006 Dec;27(36):5978-89. PubMed
  • S. Taqvi and K. Roy. Influence of scaffold physical properties and stromal cell co-culture on hematopoietic differentiation of mouse embryonic stem cells. Biomaterials 2006 Dec; 27(36):6024-31. PubMed
  • S. Taqvi, L. Dixit and K. Roy. Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells. Biomed Mater Res A. 2006 Dec 1;79(3):689-97. PubMed
  • S. P. Kasturi, H. Qin, K. S. Thomson, S. El-Bereir, S.-C. Cha, S. Neelapu, L. W. Kwak and K. Roy. Prophylactic anti-tumor effects in a B cell lymphoma model with DNA vaccines delivered on polyethylenimine (PEI) functionalized PLGA microparticles. J Control Release. 2006 Jul 20;113(3):261-70. PubMed
  • Y. Lu, G. Mapili, G Suhali, S. Chen and K. Roy. A digital micro-mirror based device for the fabrication of spatially patterned tissue engineering scaffolds. J Biomed Mater Res A. 2006 May;77(2):396-405. PubMed

2005

  • S. Kasturi, K. Sachaphibulkij and K. Roy. Covalent-conjugation of polyethyeleneimine on biodegradable polymer microparticles for delivery of plasmid DNA vaccines, Biomaterials. 2005 Nov; 26(32):6375-85. PubMed
  • G. Mapili, Y. Lu, S. Chen and K. Roy. Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds, J Biomed Mater Res B Appl Biomater. 2005 Nov;75(2):414-24. PubMed
  • H. Liu and K. Roy. Biomimetic 3D Cultures Significantly Increases Hematopoietic Differentiation Efficacy of Embryonic Stem Cells. Tissue Engineering, 2005 Jan-Feb;11 (1-2), 319-330. PubMed

2004 and earlier

  • H. Gu and K. Roy. Topical permeation enhancers significantly increase delivery of polymer micro and nanoparticles to epidermal Langerhans’ cells. Journal of Drug Delivery Science & Technology, 2004;14 (4) 265-273.
  • M. Follen, F.L. Meyskens, R.D. Alvarez, J.L. Walker, M.C. Bell, K.A. Storhz, J. Sastry, K. Roy. Richards-Kortum R, Corneilson TL. Cervical cancer chemoprevention, vaccines, and surrogate end point biomarkers. Cancer. 2003 Nov;98(9 Suppl): 2044-51. PubMed
  • K. Roy, D. Wang, M.L Hedley, S.P. Barman. Gene delivery with in-situ crosslinking polymer networks generates long-term systemic protein expression. Molecular Therapy. 2003;7(3):401-8. PubMed
  • U. McKeever, S. Barman, T. Hao, P. Chambers, S. Song, L. Lunsford, Y. Hsu, K. Roy, M.L. Hedley. Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles. Vaccine 2002;22-20(11-12):1524-153. PubMed
  • H.Q. Mao, K. Roy, V.L. Truong-Le, K.Y. Lin, K.A. Janes, Y. Wang, J.T. August, and K.W. Leong. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J. Controlled Release, 70, 399-421 (2001). PubMed
  • K. Roy, H.Q. Mao, S.K. Huang, K. W. Leong. Oral gene delivery with DNA-chitosan nanoparticles generates immunologic protection in a murine model of peanut allergy. Nature Medicine, 5 (4) 387-391 (1999). PubMed
  • K.W. Leong, H.Q. Mao, V.L. Truong-Le, K. Roy, S.M. Walsh and J.T. August. DNA nanospheres as non-viral gene delivery vehicles. J. Controlled Release, 53 (1): 183-193 (1998). PubMed
  • D.R. Ingrams, J.K. Dhingra, K. Roy, D.F. Perrault Jr., I.D. Bottrill, S. Kabani, E.E. Rebeiz, M.M. Pankratov, S.M. Shapshay, R. Manoharan, I. Itzkan, M.S. Feld. Autofluorescence characteristics of Oral Mucosa. Head and Neck 19 (1): 27-32 (1997). PubMed

Books, Chapter of Books, Editor of Books

Book Editor:

  • Biomaterials as Stem Cell Niche, Editor: K. Roy Springer, Published Fall 2010. Part of the series: Studies in Mechanobiology, Tissue Engineering and Biomaterials, Series editor: Amit Gefen

Book Chapters:

  • A. Singh, P. Pradhan, and K. Roy, (2011), Immunoengineering approaches towards combinatorial delivery of immune-modulators and antigens, In “Immune Potentiators and next generation vaccines”, Editor, M. Singh, Novartis, Springer Publications. Submitted.
  • P. Wanakule, K. Roy (2010), Drug Delivery, In Press, CRC Handbook in Biomedical Engineering, Edition: Tissue Engineering, Editor: Antonios G. Mikos and John Fischer, To be published in 2012.
  • K. Fridley, K. Roy (2010), Stem Cells and Hematopoiesis, In Press, CRC Handbook in Biomedical Engineering, Edition: Stem Cell Bioengineering, Editor: David Schaffer, To be published in 2012.
  • Singh, A, K. Roy (2010), Synthetic Niches for Stem Cell Differentiation into T cells, Editor: K. Roy, Springer, Fall 2010.
  • Kim, M.H., K. Roy (2009), Ligand-Functionalized Biomaterial Surfaces: Controlled Regulation of Signaling Pathways to Direct Stem Cell Differentiation. In: Puleo, D.A., Bizios, R., eds. Biological Interactions on Material Surfaces, Springer. pp. 158-170.
  • Roy, K (Author), Gene Delivery with BioMEMS, In “Polymeric Gene Delivery Systems”, Ed: M. Amiji, CRC Press, September 2004.

Patents and Inventions

  • Patent Filed: 61/328,339, Method of Producing Antigen-Specific Cells, Filed, April 2010.
  • Patent Filed: 61/327,168, Multi-Layered Hydrogel Constructs and Associated Methods, April 2010.
  • Patent Filed: 12/720,546, Enhancement of Polysaccharide-Mediated Nucleic Acid Delivery, March 9, 2010.
  • Patent Filed: USPTO No: 2010/031866, Hydrogels for Combinatorial Delivery of Immune- Modulating Bio Molecules, April 2010.
  • Patent filed: 61/338,634, Modified Chitosan for Delivery of Drugs or Imaging Agents, February 2010.
  • Patent Filed: USPTO No: 2010/0010102 Triggered release of drugs from polymer particles, July 9, 2009.
  • Patent Filed: USPTO 2007/0014752 Surface functionalization of polymeric materials , July 7, 2006.
  • Patent Filed: USPTO 2007/0031505 Methods for fabricating nano and microparticles for drug delivery, May 5, 2006.
  • Provisional Patent Filed: 065715.0114 (OTC-5009-Roy) Systems and Methods for the Production of Differentiated Cells, April 2005.
  • Patent Filed: USPTO 2004/0147466 Nucleic acid delivery formulations.
  • US Patent No. 6,475,995, Awarded Nov 5, 2002 Oral Delivery of Nucleic Acid Vaccines byParticulate Complexes.
  • U. S. Patent No. 5,972,707, Awarded Oct 26, 1999 Gene Delivery System.