Laboratory for Cellular and Macromolecular Engineering
P. Pradhan, J. Leleux, J. Liu, K. Roy. A simple, clinically relevant therapeutic vaccine shows long-term protection in an aggressive, delayed-treatment B lymphoma model. JCI Insight, 2017. DOI: 10.1172/jci.insight.92522.
I. Riviere and K. Roy. Perspectives on Manufacturing of High-Quality Cell Therapies. Mol Ther, 2017. DOI: 10.1016/j.ymthe.2017.04.010
A. Singh and K. Roy. Immuno-engineering: The Next Frontier in Therapeutics Delivery. Adv Drug Deliv Rev, 2017. DOI: 10.1016/j.addr.2017.08.005
C. L. Zhu, P. Pradhan, D. Huo, S. Shen, K. Roy, Y.N. Xia. Reconstitution of Low-Density Lipoproteins with Fatty Acids for the Targeted Delivery of Drugs into Cancer Cells. Angewandte Chemie-International Edition, 2017. DOI: 10.1002/anie.201704674
K. Parratt, J. Jeong, P. Qiu, K. Roy. 3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches. Lab on a Chip, 2017. DOI: 10.1039/c7lc00451f
R. G. Mannino, A. N. Santiago-Miranda, P. Pradhan, Y. Z. Qiu, J. C. Mejias, S. S. Neelapu, K. Roy, W. A. Lam. 3D microvascular model recapitulates the diffuse large B-cell lymphoma tumor microenvironment in vitro. Lab on a Chip, 2017. DOI: 10.1039/c6lc01204c
R. Madan-Lala, P. Pradhan, K. Roy. Combinatorial Delivery of Dual and Triple TLR Agonists via Polymeric Pathogen-like Particles Synergistically Enhances Innate and Adaptive Immune Responses. Scientific Reports, 2017. DOI: 10.1038/s41598-017-02804-y
J. A. Leleux, P. Pradhan, K. Roy. Biophysical Attributes of CpG Presentation Control TLR9 Signaling to Differentially Polarize Systemic Immune Responses. Cell Reports, 2017. DOI: 10.1016/j.celrep.2016.12.073
P. Jurney, R. Agarwal, V. Singh, D. Choi, K. Roy, S.V. Sreenivasan, L. Shi. Unique size and shape-dependent uptake behaviors of non-spherical nanoparticles by endothelial cells due to a shearing flow. Journal of Controlled Release, 2017. DOI: 10.1016/j.jconrel.2016.11.033
N. J. Dwarshuis, K. Parratt, A. Santiago-Miranda, K. Roy. Cells as advanced therapeutics: State-of-the-art, challenges, and opportunities in large scale biomanufacturing of high-quality cells for adoptive immunotherapies. Adv Drug Deliv Rev, 2017. DOI: 10.1016/j.addr.2017.06.005
E. Dawson, J. A. Leleux, P. Pradhan, K. Roy. Surface-Presentation of CpG and Protein-Antigen on Pathogen-Like Polymer Particles Generate Strong Prophylactic and Therapeutic Antitumor Protection. ACS Biomaterials Science & Engineering, 2017. DOI: 10.1021/acsbiomaterials.6b00384
R. Toy and K. Roy. Engineering Nanoparticles to Overcome Barriers to Immunotherapy. Bioengineering & Translational Medicine, 2016. DOI: 10.1002/btm2.10005
K.H. Roh and K. Roy. Engineering approaches for regeneration of T lymphopoiesis. Biomater Res. 2016 Jun 29;20:20. doi: 10.1186/s40824-016-0067-1. eCollection 2016. PubMed
K.H. Roh, R.M. Nerem, and K. Roy. Biomanufacturing of Therapeutic Cells: State of the Art, Current Challenges, and Future Perspectives. Annu Rev Chem Biomol Eng. 2016 Jun 7;7:455-78. doi: 10.1146/annurev-chembioeng-080615-033559. PubMed
M.R. Nelson and K. Roy. Bone-marrow mimicking biomaterial niches for studying hematopoietic stem and progenitor cells. J. Mater. Chem. B, 2016,4, 3490-3503. DOI: 10.1039/C5TB02644J
S. Lee, M. T. Nguyen, M. G. Currier, J. B. Jenkins, E. A. Strobert, A. E. Kajon, R. Madan-Lala, Y. A. Bochkov, J. E. Gern, K. Roy, X. Y. Lu, D. D. Erdman, P. Spearman, M. L. Moore. A polyvalent inactivated rhinovirus vaccine is broadly immunogenic in rhesus macaques. Nature Communications, 2016. DOI: 10.1038/ncomms12838
J. Leleux, A. Atalis, and K. Roy. Engineering immunity: Modulating dendritic cell subsets and lymph node response to direct immune-polarization and vaccine efficacy.J Control Release. 2015 Dec 10;219:610-21. doi: 10.1016/j.jconrel.2015.09.063. Epub 2015 Oct 20.
R. Agarwal, P. Jurney, M. Raythatha, V. Singh, S.V. Sreenivasan, L. Shi, K. Roy. Effect of shape, size, and aspect ratio on nanoparticle penetration and distribution inside solid tissues using 3D spheroid models.Adv Healthc Mater. 2015 Oct 28;4(15):2269-80. doi: 10.1002/adhm.201500441. Epub 2015 Sep 16.
V. Singh, R. Agarwal, K. Roy., L. Shi, S.V. Sreenivasan. Scalable Fabrication of Ultra-Soft Shape Specific Nanocarriers for Diagnostics and Drug Delivery. Journal of Micro and Nano-Manufacturing. Epub 2014 Oct; doi:10.1115/1.4028896. ASME
S. Li, K.K. Hirschi, K. Roy. Induced Pluripotent Stem Cells for Regenerative Medicine, Annu Rev Biomed Eng. 2014 Jul;16:277-94. doi: 10.1146/annurev-bioeng-071813-105108. ASME
P. Pradhan, H. Qin, J.A. Leleux, D. Gwak, I. Sakamaki, L.W. Kwak, K. Roy. The effect of combined IL10 siRNA and CpG ODN as pathogen-mimicking microparticles on Th1/Th2 cytokine balance in dendritic cells and protective immunity against B cell lymphoma. Biomaterials. 2014 Jul; 35(21):5491-504. doi: 10.1016/j.biomaterials.2014.03.039. PubMed
A. Purwada, K. Roy, A. Singh. Engineering vaccines and niches for immune modulation, Acta Biomaterialia. 2014, Apr:10(4):1728-1740.doi:10.1016/j.actbio.2013.12.036. PubMed
J.S. Lewis, K. Roy, B.G. Keselowsky. Materials that harness and modulate the immune system, MRS Bulletin 39 (01), 25-34. CJO
I. Fernandez, T.P. Ooi, K. Roy. Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous notch and tetramer-TCR signaling. Stem Cells. 2014 Jan; 32(1). doi: 10.1002/stem.1512. PubMed
P. Jurney, R. Agarwal, V. Singh, K. Roy., S.V. Sreenivasan, L. Shi. Size-Dependent Nanoparticle Margination and Adhesion Propensity in a Microchannel. Journal of Nanotechnology in Engineering and Medicine. 2013 Nov;4(3). doi: 10.1115/1.4025609. ASME
R. Agarwal, V. Singh, P. Jurney, S.V. Sreenivasan, L. Shi, K. Roy. Cells Prefer Hydrogel Nanodiscs over Nanorods and Use Shape-specific Uptake Mechanisms. Proc Natl Acad Sci USA. 2013 Oct;110(43):17247-52. doi: 10.1073/pnas.1305000110. PNAS
R. Agarwal, K. Roy. Intracellular Delivery of Polymeric Nanocarriers: A Matter of Size, Shape, Charge, Elasticity and Surface Composition. , Therapeutics Delivery. 2013 Jun;4(6):705-23. doi: 10.4155/tde.13.37. Pubmed
J. Leleux, K. Roy. Micro and nanoparticle-based delivery systems for vaccine immunotherapy: an immunological and materials perspective. Adv Healthc Mater. 2013 Jan;2(1):72-94. doi: 10.1002/adhm.201200268. Pubmed
I. Fernandez, K. Fridley, D. Arasappan, R. Ambler, P.W. Tucker, K. Roy. Gene expression profile and functionality of ESC-derived Lin-ckit+Sca-1+ cells are distinct from Lin-ckit+Sca-1+ cells isolated from fetal liver or bone marrow. PLoS One. 2012;7(12) doi: 10.1371/journal.pone.0051944. PubMed
P. Wanakule, G.W. Liu, A.T. Fleury, K. Roy. Nano-inside-micro: Disease-responsive microgels with encapsulated nanoparticles for intracellular drug delivery to the deep lung. Journal of Controlled Release. 2012 Jul;162(2):429-437. doi: 10.1016/j.jconrel.2012.07.026. PubMed
R. Agarwal, V. Singh, P. Jurney, L. Shi, S.V. Sreenivasan, K. Roy. Scalable imprinting of shape- specific polymeric nanocarriers using a release layer of switchable water solubility. ACS Nano. 2012 Mar; 6(3):2524-2531. doi: 10.1021/nn2049152. PubMed
P. Wanakule, K. Roy. Disease-Responsive Drug Delivery: The Next Generation of Smart Delivery Devices. Current Drug Metabolism, Volume 13, Number 1, January 2012, pp. 42-49(8). PubMed
L. Nguyen, A. Kudva, N. Saxena and K. Roy. Engineering articular cartilage with spatially varying matrix composition and mechanical properties from a single stem cell population using a multi- layered hydrogel. Biomaterials. 2011 Oct; 32(29):6946-52. doi: 10.1016/j.biomaterials.2011.06.014. PubMed
A. Singh, H. Qin, I. Fernandez, J. Wei, J. Lin, L. W. Kwak and K. Roy. An injectable synthetic immune-priming center mediates efficient T-cell class switching and T-helper 1 response against B cell lymphoma. Journal of Controlled Release. 2011 Oct;155(2):184-92 doi: 10.1016/j.jconrel.2011.06.008. PubMed
M Caldorera-Moore, MK Kang, Z Moore, V Singh, SV Sreenivasan, L Shi, R Huang and K Roy. Swelling behavior of nanoscale, shape- and size-specific, hydrogel particles fabricated using imprint lithography. Soft Matter. 2011; 7:2879-2887. RSC
J Lin, I Fernandez, K Roy. Development of Feeder-Free Culture Systems for Generation of ckit+sca1+ Progenitors from Mouse iPS Cells. Stem Cell Rev. 2011 Sep;7(3):736-47. doi: 10.1007/s12015-010-9215-5. (J Lin and I Fernandez contributed equally to this work). PubMed
K Fridley, I Fernandez, M-T A Li, RB Kettlewell, K Roy. Unique Differentiation Profile of Mouse Embryonic Stem Cells in Rotary and Stirred Tank Bioreactors. Tissue Engineering Part A. 2010 Nov;16(11):3285-98. doi: 10.1089/ten.TEA.2010.0166. PubMed
B Ghosn, A Singh, M Li, AV Vlassov, C Burnett, N Puri, K Roy. Efficient gene silencing in lungs and liver using imidazole-modified chitosan as a nanocarrier for small interfering RNA. Oligonucleotides.2010 Jun;20(3):163-72. doi: 10.1089/oli.2010.0235. PubMed
J Lin, H Nie, PW Tucker, K Roy. Controlled Major Histocompatibility Complex-T Cell Receptor Signaling Allows Efficient Generation of Functional, Antigen-Specific CD8(+) T Cells from Embryonic Stem Cells and Thymic Progenitors. Tissue engineering Part A. 2010 Sep;16(9):2709-20. doi: 10.1089/ten.TEA.2009.0707. PubMed
M Caldorera-Moore, N Guimard, L Shi, K Roy. Designer nanoparticles: incorporating size, shape and triggered release into nanoscale drug carriers. Expert Opin Drug Deliv. 2010 Apr;7(4):479-95. doi: 10.1517/17425240903579971. PubMed
I Kadiyala, Y Loo, K Roy. J Rice, KW Leong. Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes. Eur J Pharm Sci. 2010 Jan 31;39(1-3):103-9. doi: 10.1016/j.ejps.2009.11.002. PubMed
B Ghosn, AL van de Ven, J Tam, A Gillenwater, KV Sokolov, R Richards-Kortum, K Roy. Efficient mucosal delivery of optical contrast agents using imidazole-modified chitosan. J Biomed Opt. 2010 Jan-Feb;15(1):015003. doi: 10.1117/1.3309739. PubMed
A Singh, S Suri, K Roy. In-situ crosslinking hydrogels for combinatorial delivery of chemokines and siRNA-DNA carrying microparticles to dendritic cells. Biomaterials. 2009 Oct;30(28):5187-200. doi: 10.1016/j.biomaterials.2009.06.001. PubMed
A. Singh, H. Nie, B. Ghosn, H. Qin, L. W. Kwak, K. Roy. Efficient modulation of T cell response by dual-mode, single carrier delivery of cytokine-targeted siRNA and DNA vaccine to antigen presenting cells. Molecular Therapy. 2008 Dec;16(12):2011-21. doi: 10.1038/mt.2008.206. PubMed
E. Dawson, G. Mapili, K. Erickson, S. Taqvi and K. Roy. Biomaterials for Stem Cell Differentiation. Adv Drug Delivery Review. 2008 Jan 14;60(2):215-28. PubMed
B. Ghosn, S. Kasturi and K. Roy. Enhancing Polysaccharide-mediated Delivery of Nucleic Acids Through Functionalization with Secondary and Tertiary Amines. Current Topics in Medicinal Chemistry. 2008;8(4):331-40. PubMed
L.C. Glangchai, M.C. Moore, L. Shi and K. Roy. Nanoimprint Lithography Based Fabrication of Shape-specific, Enzymatically-Triggered Smart Nanoparticles. Journal of Controlled Release. 2008 Feb 11;125(3):263-72. PubMed
L.H. Han, G. Mapili, S. Chen, and K. Roy. Projection micro-printing of 3-dimensional scaffolds for tissue engineering. Journal of Manufacturing Science and Engineering, 2007
L. Brannon-Peppas, B. Ghosn, K. Roy, K. Cornetta. Encapsulation of nucleic acids and opportunities for cancer treatment. Pharm Res. 2007 Apr;24(4):618-27. PubMed
H. Liu, J. Lin and K. Roy. Effect of 3D scaffold and dynamic culture condition on the global gene expression profile of mouse embryonic stem cells. Biomaterials. 2006 Dec;27(36):5978-89. PubMed
S. Taqvi and K. Roy. Influence of scaffold physical properties and stromal cell co-culture on hematopoietic differentiation of mouse embryonic stem cells. Biomaterials 2006 Dec; 27(36):6024-31. PubMed
S. Taqvi, L. Dixit and K. Roy. Biomaterial-based notch signaling for the differentiation of hematopoietic stem cells into T cells. Biomed Mater Res A. 2006 Dec 1;79(3):689-97. PubMed
S. P. Kasturi, H. Qin, K. S. Thomson, S. El-Bereir, S.-C. Cha, S. Neelapu, L. W. Kwak and K. Roy. Prophylactic anti-tumor effects in a B cell lymphoma model with DNA vaccines delivered on polyethylenimine (PEI) functionalized PLGA microparticles. J Control Release. 2006 Jul 20;113(3):261-70. PubMed
Y. Lu, G. Mapili, G Suhali, S. Chen and K. Roy. A digital micro-mirror based device for the fabrication of spatially patterned tissue engineering scaffolds. J Biomed Mater Res A. 2006 May;77(2):396-405. PubMed
S. Kasturi, K. Sachaphibulkij and K. Roy. Covalent-conjugation of polyethyeleneimine on biodegradable polymer microparticles for delivery of plasmid DNA vaccines, Biomaterials. 2005 Nov; 26(32):6375-85. PubMed
G. Mapili, Y. Lu, S. Chen and K. Roy. Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds, J Biomed Mater Res B Appl Biomater. 2005 Nov;75(2):414-24. PubMed
H. Liu and K. Roy. Biomimetic 3D Cultures Significantly Increases Hematopoietic Differentiation Efficacy of Embryonic Stem Cells. Tissue Engineering, 2005 Jan-Feb;11 (1-2), 319-330. PubMed
2004 and earlier
H. Gu and K. Roy. Topical permeation enhancers significantly increase delivery of polymer micro and nanoparticles to epidermal Langerhans’ cells. Journal of Drug Delivery Science & Technology, 2004;14 (4) 265-273.
M. Follen, F.L. Meyskens, R.D. Alvarez, J.L. Walker, M.C. Bell, K.A. Storhz, J. Sastry, K. Roy. Richards-Kortum R, Corneilson TL. Cervical cancer chemoprevention, vaccines, and surrogate end point biomarkers. Cancer. 2003 Nov;98(9 Suppl): 2044-51. PubMed
K. Roy, D. Wang, M.L Hedley, S.P. Barman. Gene delivery with in-situ crosslinking polymer networks generates long-term systemic protein expression. Molecular Therapy. 2003;7(3):401-8. PubMed
U. McKeever, S. Barman, T. Hao, P. Chambers, S. Song, L. Lunsford, Y. Hsu, K. Roy, M.L. Hedley. Protective immune responses elicited in mice by immunization with formulations of poly(lactide-co-glycolide) microparticles. Vaccine 2002;22-20(11-12):1524-153. PubMed
H.Q. Mao, K. Roy, V.L. Truong-Le, K.Y. Lin, K.A. Janes, Y. Wang, J.T. August, and K.W. Leong. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J. Controlled Release, 70, 399-421 (2001). PubMed
K. Roy, H.Q. Mao, S.K. Huang, K. W. Leong. Oral gene delivery with DNA-chitosan nanoparticles generates immunologic protection in a murine model of peanut allergy. Nature Medicine, 5 (4) 387-391 (1999). PubMed
K.W. Leong, H.Q. Mao, V.L. Truong-Le, K. Roy, S.M. Walsh and J.T. August. DNA nanospheres as non-viral gene delivery vehicles. J. Controlled Release, 53 (1): 183-193 (1998). PubMed
D.R. Ingrams, J.K. Dhingra, K. Roy, D.F. Perrault Jr., I.D. Bottrill, S. Kabani, E.E. Rebeiz, M.M. Pankratov, S.M. Shapshay, R. Manoharan, I. Itzkan, M.S. Feld. Autofluorescence characteristics of Oral Mucosa. Head and Neck 19 (1): 27-32 (1997). PubMed
Books, Chapter of Books, Editor of Books
Biomaterials as Stem Cell Niche, Editor: K. Roy Springer, Published Fall 2010. Part of the series: Studies in Mechanobiology, Tissue Engineering and Biomaterials, Series editor: Amit Gefen
A. Singh, P. Pradhan, and K. Roy, (2011), Immunoengineering approaches towards combinatorial delivery of immune-modulators and antigens, In “Immune Potentiators and next generation vaccines”, Editor, M. Singh, Novartis, Springer Publications. Submitted.
P. Wanakule, K. Roy (2010), Drug Delivery, In Press, CRC Handbook in Biomedical Engineering, Edition: Tissue Engineering, Editor: Antonios G. Mikos and John Fischer, To be published in 2012.
K. Fridley, K. Roy (2010), Stem Cells and Hematopoiesis, In Press, CRC Handbook in Biomedical Engineering, Edition: Stem Cell Bioengineering, Editor: David Schaffer, To be published in 2012.
Singh, A, K. Roy (2010), Synthetic Niches for Stem Cell Differentiation into T cells, Editor: K. Roy, Springer, Fall 2010.
Kim, M.H., K. Roy (2009), Ligand-Functionalized Biomaterial Surfaces: Controlled Regulation of Signaling Pathways to Direct Stem Cell Differentiation. In: Puleo, D.A., Bizios, R., eds. Biological Interactions on Material Surfaces, Springer. pp. 158-170.
Roy, K (Author), Gene Delivery with BioMEMS, In “Polymeric Gene Delivery Systems”, Ed: M. Amiji, CRC Press, September 2004.
Patents and Inventions
Patent Filed: 61/328,339, Method of Producing Antigen-Specific Cells, Filed, April 2010.
Patent Filed: 61/327,168, Multi-Layered Hydrogel Constructs and Associated Methods, April 2010.
Patent Filed: 12/720,546, Enhancement of Polysaccharide-Mediated Nucleic Acid Delivery, March 9, 2010.
Patent Filed: USPTO No: 2010/031866, Hydrogels for Combinatorial Delivery of Immune- Modulating Bio Molecules, April 2010.
Patent filed: 61/338,634, Modified Chitosan for Delivery of Drugs or Imaging Agents, February 2010.
Patent Filed: USPTO No: 2010/0010102 Triggered release of drugs from polymer particles, July 9, 2009.
Patent Filed: USPTO 2007/0014752 Surface functionalization of polymeric materials , July 7, 2006.
Patent Filed: USPTO 2007/0031505 Methods for fabricating nano and microparticles for drug delivery, May 5, 2006.
Provisional Patent Filed: 065715.0114 (OTC-5009-Roy) Systems and Methods for the Production of Differentiated Cells, April 2005.